Pretreatment to Increase Hydrogen Producing Bacteria (HPB)
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چکیده
This Chapter focuses on the investigation of an easy and efficacious method of obtaining a hydrogen-producing bacteria (HPB) culture to the detriment of hydrogen-consuming bacteria (HCB), such as methanogens and homoacetogenic bacteria. Although the use of mixed microflora is more viable from both the practical and biological points of view, important limitations arise from the co-activity of HPB and HCB. In this respect, pretreatment is one of the most important issues in anaerobic hydrogen production, in order to produce suitable inocula of HPB. In particular, we investigated the effectiveness of acid pretreatment applied to mixed microflora in order to stop methanogen activity. We evaluated the content of Clostridium bacteria, which are the main ones responsible for H2 fermentation in two of the most widely used inoculum sources: anaerobic sludge from wastewater treatment plants and rumen microorganisms from cow stomachs.
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